Origin of subgenomes in the circumboreal allopolyploid carnivorous plant Drosera anglica (Droseraceae)

Premise of Study
The parentage of one of the most widespread members of the carnivorous sundew genus Drosera, the enigmatic allopolyploid Drosera anglica, remains uncertain despite over 100 years of morphological, cytological, and more recently, molecular study.
Methods
Using transcriptomic and genomic data from 12 species and 20 populations across Drosera sect. Drosera including four D. anglica populations and a disjunct Idaho population of D. intermedia, we carried out reference-based assembly in HybPiper and phased sequences in HybPhaser. We estimated a species tree and quantified gene tree discordance; we calculated heterozygosity statistics and pairwise divergence between individuals. In addition to genome-wide analyses, we extracted and assembled rbcL and ITS reads from genome and transcriptome datasets to compare to previous Sanger sequencing data. We also generated flow cytometry data to verify the ploidy levels of D. anglica and D. rotundifolia populations.
Key Results
Seq..., , , This repository contains data and analysis files for Mohn, RA, Yang, Y. Origin of subgenomes in the circumboreal allopolyploid carnivorous plant Drosera anglica (Droseraceae).

### Drosera_anglica_Dryad_Nov2025.zip

The full dataset

### Dspat_6443_refgenes.fa

The 6443 reference genes from D. spatulata used for input to HybPiper

The genes are labeled with their Drosera spatulata names as published in Palfalvi et al., 2020 Current Biology. [https://doi.org/10.1016/j.cub.2020.04.051](https://doi.org/10.1016/j.cub.2020.04.051)
They are in a fasta format.

The complete list of Drosera spatulata genes were obtained here: [https://www.biozentrum.uni-wuerzburg.de/carnivorom/resources/](https://www.biozentrum.uni-wuerzburg.de/carnivorom/resources/)

### finalAlnPreCleaning

The alignments of recovered genes after phasing of D. anglica subgenomes before trimming with Phyx

This zipped folder contains a separate file for each gene, titled by the D. spatulata name. All samples with that gene are..., , **Changes after Mar 10, 2025:**

Nov 2025: Added the folder rbcL_and_ITS and frozen copies of scripts used in analyses.