Next Generation Sequencing Facilitates Quantitative Analysis of Yeast Cells Treated with and without Proanthocyanidins

Proanthocyanidins (PAs) could pose significant enhancement on the metabolism and fermentation efficiency of Saccharomyces cerevisiae yeast cells, which has been elucidated from metabolic level and cell phenotype in our previous work. The goals of this study are to compare the transcriptome profiling (RNA-seq) of yeast cells treated with and without PAs to explore the possible global protection mechanism. After treated with 0 g/L, 0.1g/L, 1.0 g/L PAs, the total RNA of Saccharomyces cerevisiae strain AWRI R2 was extracted and sequenced at Illumina HiSeq sequencing platform. The analysis yielded a total of 524025062 clean sequencing reads. The clean reads were processed for the following bioinformatics analysis. Overall design: Saccharomyces cerevisiae strain AWRI R2 was cultivated in model synthetic medium (MSM) supplemented with 0 g/L, 0.1g/L, 1.0 g/L PAs (marked as group A, group B and group C, respectively). The total RNA was extracted and sequencing library was prepared after quantity and quality evaluation. Illumina HiSeq sequencing platform was employed for the library sequencing at GENEWIZ (Suzhou, China). The sequencing data were processed in the following bioinformatics analysis after reads filtration.