Super-enhancer LncRNA expression profile in human bladder cancer cell line and human urothelial immortalized cell line

To further explore the differential expression profile of super-enhancer long noncoding RNA (LncRNA) in human bladder cancer, we have employed super-enhancer lncRNA microarray expression profiling as a discovery platform to identify potential differential expression profile of super-enhancer lncRNA between human bladder cancer cell (UM-UC-3 cell) and urothelial immortalized cell (SV-HUC-1 cell). Results showed that a large number of differentially expressed super-enhancer lncRNA were found between UM-UC-3 cell and SV-HUC-1 cell. In this study, We verified 5 up-regulated differentially expressed super-enhancer lncRNAs using qPCR, of which the highest fold change is LINC00162. Then we further explored the biological function and mechanism of LINC00162 in bladder cancer in this study. In the initial stage, we conducted a super-enhancer LncRNA microarray analysis using human urothelial immortalized SV-HUC-1 cells as a control group and human bladder cancer UM-UC-3 cells as an experimental group. Then we find out the differentially expressed super-enhancer LncRNAs, and explore its function and mechanism of action through molecular biology experiments in vivo and in vitro.