SmcHD1 genome-wide occupancy

To identify SmcHD1 genome-wide occupancy relative to global level of DNA methylation, we generated anti-SmcHD1 antiserum and used it in chromatin immunoprecipitation assay. We used SH-SY5Y neurobalstoma cells treated with DNA demethylating agent 5azaC for 72 hours to induce global loss of DNA methylation. The protein level of maintenance DNA methyltransferase dnmt1 was significatly reduced upon 5azaC treatment. The 5azaC treated and non-treated cells were then used for chromatin immunoprecipitation. Examination of SmcHD1 genome occupancy in SH-SY5Y cells treated with 5azaC compared with non-treated cells