Oxidative stress-mediated alterations of the microRNA expression profile in porcine granulosa cells using RNA-Seq technique

To investigate the effects of oxidative stress induced by hydrogen peroxide(H2O2) on miRNA expression in porcine granulosa cells,we examined the impact of H2O2-mediated oxidative stress in procine GC through miRNA-Seq. We identified 33 (19 upregulated, 14downregulated) and 22 (14 upregulated, 8 downregulated miRNAs) differentially expressed miRNAs (DEmiRNAs) at 300μM and 100μM H2O2, respectively, compared with the control group. miRNA profiles of control and H2O2 treatment groups (100 and 300μM) were generated by deep sequencing using Illumina HiSeqTM 2500