Dataset for the article "Electron-Transfer-Mediated by Detonation Nanodiamonds for Enhanced Cortisol Immunoassay"

Dataset for the article "Electron-Transfer-Mediated by Detonation Nanodiamonds for Enhanced Cortisol Immunoassay".

Chakavak Esmaeili1*, Jaroslav Kuliček1, Markéta Šlapal Bařinková1, Bohuslav Rezek1*

1 Faculty of Electrical Engineering, CTU in Prague, Technicka 2, 16627 Prague, Czech Republic

Figure 1. The preparation and immobilization step of the cortisol fabrication based on electrochemical immunosensor Figure 2. 3D AFM images  of (1) carbon bare electrode, (2) carbon electrode /DND, (3) carbon electrode/DND/EDC NHS (4) carbon electrode/ DND /EDC NHS/Ab, (5) carbon electrode/ DND /EDC NHS/Ab /BSA, (6) carbon electrode/ DND /EDC NHS/Ab/BSA/AgFigure 3. Raman spectra of immunosensor compositionFigure 4.  FTIR spectra of DND (gray line), DND/ EDC NHS (blue line), DND/EDC NHS/Ab (green line). All spectra were recorded in the region between 4000–800 cm−1 with a resolution of 4 cm− 1 and 128 scansFigure 5. Functionalization step. (1) carbon bare electrode, (2) carbon electrode /DND, (3) carbon electrode /DND/EDC NHS, (4) carbon electrode/DND/ EDC NHS/Ab and sensing step (5) carbon electrode/DND/ EDC NHS/Ab/BSA and (6) carbon electrode/DND/ EDC NHS/Ab/BSA/Ag in the presence of 100 nM cortisol in pH 7.0, containing 250 mM KCl and 5 mM K3[Fe(CN)6] and 5 mM K4[Fe(CN)6], Scan rate 0.1Vs−1Figure 6. (a) Calibration curve of DPV with varying Ag concentrations. (b) Sensitivity comparison between configurations without Ab receptor (carbon electrode/DND/EDC NHS/Ag) and with Ab receptor (carbon electrode/DND/EDC NHS/Ab/BSA/Ag) in PBS solution, pH 7.0, containing 250 mM KCl and 5 mM K3[Fe (CN)6] and 5 mM K4[Fe (CN)6]Figure 7. Interference study involving progesterone (2430 pg/ml), β-oestradiol (32 pg/ml), cortisone (28.6 nM), and corticosterone (3.94 nM) with respect to cortisol (100nM)Figure 8. Stability of the immunosensor with repeated measurements of cortisol

Figure S1.  Optical images obtained for (1) bare carbon electrode, (2) DND, (3) DND/EDC NHS, (4) DND/EDC NHS/Ab, (5) DND/EDC NHS/Ab/BSA, (6) DND/EDC NHS/Ab/BSA/Ag.Figure S2. SEM images of (1) bare carbon SPE, (2) carbon electrode/ DND, (3) carbon electrode/ DND /EDC NHS, (4) carbon electrode/ DND /EDC NHS/Ab, (5) carbon electrode/DND/EDC NHS/Ab/BSA, (6) carbon electrode/ DND /EDC NHS/Ab/BSA/AgFigure S3. (a) CV and (b) DPV of carbon bare electrode, carbon electrode /EDC NHS, carbon electrode/EDC NHS/Ab, carbon electrode/EDC NHS/Ab/BSA, carbon electrode/EDC NHS/Ab/BSA/Ag, in the presence of 100 nM cortisol,  pH 7.0, containing 250 mM KCl and 5 mM K3[Fe(CN)6] and 5 mM K4[Fe(CN)6], Scan rate 0.1Vs−1.

Table 1. Determination of cortisol in artificial saliva sample (n = 3)Table S1. Surface roughness of immunosensors