Table S1. The statistical difference of 28 miRNAs between 5 IDC pools and 3 HCs pools at the screening cohort

Abstract:

Background: Breast
cancer, especially invasive ductal carcinoma (IDC), is the cause of a great
clinical burden. MicroRNA (miRNA) could be considered as a non-invasive
biomarkers for IDC diagnosis.

Methods: 260
participants (135 IDC patients and 125 healthy controls (HCs)) were enrolled
in a three-cohort study. The expression of 28 miRNAs in serum were detected
with quantitative reverse transcription-polymerase chain reaction (qRT-PCR).
Bioinformatic analysis was used for predicting the target genes of 3 selected
miRNAs..

Results: The
expression level of 7 miRNAs (miR-9-5p, miR-34b-3p, miR-1-3p, miR-146a-5p,
miR-20a-5p, miR-34a-5p, miR-125b-5p) were discrepant at the validation
cohort. Through statistical test, a three-miRNA panel (miR-9-5p, miR-34b-3p,
miR-146a-5p) was significant for IDC diagnosis (AUC=0.880, sensitivity =
86.25%, specificity = 81.25%).

Conclusion: The three-miRNA panel in serum could
be used as a non-invasive biomarker in the diagnosis of IDC