single cell RNA sequencing of hematopoietic stem cells aged in old and young niches
收藏干细胞与再生医学数据中心2022-02-20 更新2024-03-06 收录
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http://data.iscr.ac.cn/Article?id=23f2b1faddac363c1853ce525780324a
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Purpose: Compare the transcriptome of hematopoietic stem cells (HSCs) that were aged in old and young nichesMethods: barcoded GFP+ HSCs were FACS-sorted from a) three recipient mice 15 months post transplantation, and b) six serial transplantation recipient mice 5 months after the 8th transplantation, then subjected to processed using the Chromium Single-cell 3â² v2 Library Kit (10Ã Genomics, Pleasanton, CA) following the manufacturerâs instructionsResults: we obtained transcriptomes of about 12k HSCs aged in young niche, and about 10k HSCs aged in old niche, with the average sequencing depth at close to 50k reads per cellConclusions: we identified striking differences in gene expression profiles 1) between HSCs aged in young niches from mice with early aging and from mice with delayed aging, and 2) between HSCs aged in old niches and young niches when mice exhibited hematopoietic aging phenotype
提供机构：
university of southern california
创建时间：
2022-02-20
