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Mass spectrometry–based identification of potential phosphorylation sites on SPINDOC

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FLAG-tagged SPINDOC was overexpressed in HEK293T cells, which were subsequently synchronized at M phase or G1/S phase. Cells were harvested and FLAG-SPINDOC was enriched by anti-FLAG immunoprecipitation, followed by mass spectrometry–based identification of potential phosphorylation sites.

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2026-05-21
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