Whole-genome bisulfite sequencing and histone 3.3 ChIP-seq of microdissected developing mouse lens

Using whole genome bisulfite sequencing (WGBS), we investigated dynamics of DNA methylation and chromatin changes during mouse lens fiber and epithelium differentiation between embryos (E14.5) and newborns (P0.5) using microdissected lenses. Histone H3.3 variant chromatin landscapes were also generated by ChIP-seq using microdissected newborn lenses. Overall design: Whole-genome bisulfite sequencing (WGBS) of DNA methylation in microdissected mouse lens epithelium and fiber in embryonic (E14.5) and newborn (P0.5) mice, and histone 3.3 ChIP-seq of microdissected lens epithelium and fiber in newborn mice (P1)