Tissue-specific study across the stem reveals the molecular fingerprint of bark.

As a highly specialized array of tissues, bark is known to have important roles in plant protection and development. In this study we focus to understand the molecular mechanism and fingerprint of multiple pathways involve in certain biochemical processes in bark's of Betulaceae family. To this end, we have isolated bark tissues from Betula pendula and Alnus glutinosa. Using the cryosectioning technique, eight tissue types are isolated from B. pendula's bark, and two from A. glutinosa. For RNAseq library preparation, total RNA are isolated, from every fraction individually (three biological replicate for every fraction). Differential expression analyzes are conducted among -inter and -intra species tissue type. The results from gene expression analyzes are combined with genomics information, chemical and spectroscopy measurements in order to infer certain evolutionary adoptions and characteristics of barks in Betulaceae family.