Transcriptome analysis among young and senescent fibroblasts expressing shLuciferase or shFbxo22. Transcriptome analysis among young and senescent fibroblasts expressing shLuciferase or shFbxo22

We carried out the analysis using human skin fibrobllast HCA2 (MJ90) cells dox-inducible shLuciferase or shFbxo22. Cells were indcued to be senescent cells by treatment with 10 Gy. As a result, Gene set enrichment analysis revealed a significant increase in the expression of lysomal-related and autophagosomal genes in control cells, whereas this gene ontology was not increased in cells depleted of Fbxo22 Overall design: Examination of young and senescent HCA2 cells expressing dox-inducible shLuciferase or shFbxo22.