NK cell analysis in the griddient

HCT-116 cells (blue) were seeded at 30 million cells/mL in a collagen solution. A biopsy of the gel was isolated, and the cavity was filled with a hydrogel containing NK-92 cells (green). IP was added into the media to visualize dead cells. Images was obtained in a fluorescence microscope at day 0 and 15 after the addition of NK-92 cells. Quantification of the NK cell number and area covered by NK cell at day 0 and 15. Data was normalized by area covered at day 0 and plot as as mean ±SEM, n=3 . .