five

ScRNAseq analysis of EC sensitized mouse skin

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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE194254
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We performed single-cell RNA-seq to transcriptionally characterize the populations and gene expression in the skin of mice epicutaneously sensitized Balb/c mice were epicutaneously sensitized for 10 days. 2 days later the mouse skin was collected and enzymatically digested to generate a sjingle cell suspension. Barcoding and library preparation were done using the 10x Genomics Chromium platform, with the Chromium Single Cell 3′ Library & Gel Bead Kit v2 and Chromium Single Cell 3′ Chip kit v2 according to the manufacturer’s instructions.
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2022-05-10
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