High-Resolution temporal profiling of developing pancreas uncovers the mechanisms of endocrine cell fate determination [Drop-seq]

Drop-sequencing of the whole e14.5 and e16.5 mouse pancreas Three litters and 39 mouse embryonic day 14.5 pancreata was dissociated to single cells. 15,228 single cells were sequenced using Drop-seq. For e16.5, three litters and 31 embryos were used and 2,006 transcriptomes were recovered by Drop-seq.本数据集为针对全胚胎发育第14.5天（embryonic day 14.5，简写E14.5）与第16.5天（E16.5）的小鼠全胰腺所开展的Drop-seq测序（Drop-sequencing）数据。其中E14.5组实验：使用3窝孕鼠，共获取39份该时期的小鼠胰腺组织并解离为单个细胞，随后通过Drop-seq测序技术对15228个单细胞完成转录组测序；E16.5组实验：使用3窝孕鼠共31枚胚胎，通过Drop-seq测序共回收得到2006个单细胞转录组。