The data of study about the chitosan-selenium nanoparticles on depression-like behaviour induced by fluoride in mice via JAK2-STAT3 pathway

These data demonstrated that 1 mg/kg·bw CS-SeNPs mitigated fluorine-induced cortical pathological structural (Raw data 25) and functional damage (Raw data 26), abnormal activation of microglia (Raw data 27-32), and enhanced nuclear localization of P-STAT3 (Raw data 33). Raw data 25 and 26 revealed that fluoride induced obviously bigger average area of vacuolation, further the significantly reduced number of cortical surviving neurons. With 1 mg/kg·bw of CS-SeNPs supplementation, the vacuolation damage and the reduced Nissl body in the fluoride group was improved, suggesting that appropriate concentration of CS-SeNPs alleviated the oxidative stress further promoted the survival of neurons through antagonizing the neurotoxicity of fluoride. Raw data 27-29 demonstrated that fluoride could activate microglia in cortex mainly manifested as reduced number of ramified microglia and increased number of amoeboid microglia. Raw data 30-32 showed that F treatment lowered the Ramification Index, the average branch length and raised the Solidity index. After 2 mg/kg·bw CS-SeNPs treatment alone, the Ramification Index was significantly reduced, and the Solidity was significantly increased, indicating that the supplement of 2 mg/kg·bw CS-SeNPs caused the overactivation of microglia to a certain extent in Raw data 30-32. After the fluorosis mice were gavaged with 1 mg/kg·bw of CS-SeNPs, the number of ramified microglia in the cortex increased, amoeboid microglia decreased (Raw data 27-29), and average branch length, Solidity, and Ramification Index parameters recovered to normal levels (Raw data 30-32). Raw data 27-32 showed that F and 2 mg/kg·bw CS-SeNPs could cause the excessive activation of microglia, and intragastric administration of 0.5, 1 mg/kg·bw CS-SeNPs can inhibit the abnormal activation of microglia by restoring the characteristics of Solidity and Ramification Index of microglia. Raw data 33 demonstrated that the p-STAT3 nuclear localization of mice microglia induced by fluoride was increased by 42.54%±0.499. However, the increased p-STAT3 nuclear localization ratio in the F group was blocked by the supplementation of CS-SeNPs at 1 mg/kg·bw. These data provide pathological data to support the neurotoxicity of high doses of nano-selenium (2 mg/kg·bw CS-SeNPs). The lower dose of 0.5 mg/kg·bw CS-SeNPs still provided a safe threshold for long-term consumption of nano-selenium.