Expression data from Saccharomyces cerevisiae F12 cells in the presence of insoluble solids at high concentration

The implementation of a cost-effective lignocellulosic ethanol production requires developing efficient high gravity processes (i.e. working at high substrate concentrations). During the fermentation processes, the presence of high concentration of insoluble solids leads to lower glucose consumption rates, reduced ethanol volumetric productivities, and the accumulation of intracellular reactive oxygen species (ROS). Major repressed biological processes include cell cycle progression, trehalose and glycogen biosynthesis, DNA repair mechanisms, and certain genes involved in the general cell stress response. On the other hand, genes related to the glutathione, thioredoxin and methionine scavenging systems are induced. S. cerevisiae F12 cells were collected during fermentation in presence of insoluble solids for RNA extraction and hybridization on Affymetrix microarrays. Furthermore, cells collected during fermentation in absence of insoluble solids at the same time point were used as control.