Viable Cell Barcoding with SeMal and TeMal
收藏NIAID Data Ecosystem2026-05-02 收录
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资源简介：
Pooled Antibody Staining of Viable PBMC after SeMal/TeMal 21-plex Barcoding. Twenty-one individual PBMC samples (PBMC from seven separate donors with 3 replicates per donor) were barcoded using the SeMal/TeMal isotope scheme shown in Figure 5A and Supplemental Figure S5A of publication 24-120.R1 Cytometry Part A. The barcoded samples were mixed, viability stained then incubated, as a pool, with a pre-titrated 33-marker antibody cocktail (See Supplemental Table S1 for list of markers and metals). After surface marker staining, cells were fixed with 1.6% paraformaldehyde and incubated with iridium overnight.
Conclusion:
Each donor sample replicate retained its unique phenotypic profile with excellent reproducibility.
Notes:
Data used in Figure 6 of publication 24-120.R1 Cytometry Part A. This is a bead normalized fcs file.
创建时间：
2024-10-01
