METTL5, an 18S rRNA-specific m6A methyltransferase, modulates expression of stress response genes
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https://www.ncbi.nlm.nih.gov/sra/SRP223527
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Input mRNAs and ribosome protected fragments (RPFs) from wildtype and Mettl5 knockout (KO) B16 cells were analyzed by TruSeq Ribo Profile (Illumina) library preparation and high throughput sequencing Overall design: Mouse melanoma B16 cells were grown in standard conditions (DMEM + 10% FBS + Pen/Strep (100U / mL)). Input mRNAs and RPFs were isolated, ribosomal RNAs were depleted and cDNA libraries were prepared according to the TruSeq Ribo Profile pipeline (Illumina)
创建时间:
2020-04-29



