HyperTRIBE uncovers SYNCRIP RNA binding activity in hematopoietic stem cells (HSCs) and multipotent progenitors (MPPs)

Identifying SYNCRIP RNA-binding targets in HSC and MPP populations, in order to uncover which direct targets influence SYNCRIP's role in maintaining HSCs self renewal capacity. HSCs (Lin-, Sca1+, cKit+, CD150+ CD48-) and MPPs (Lin-, Sca1+, cKit+, all remaining CD150 CD48 cells) from C57BL/6 mice (The Jackson Laboratory, USA) were FACS sorted and transduced with virus expressing either empty vector (MSCV-IRES-GFP) or SYNCRIP-ADAR. 48hrs post transduction cells were sorted for GFP+ expression and used for RNA-seq.