miRNA expression data from HPC-EVs

EVs derived from HPCs could affect the progression of S. japonicum-induced fibrosis. Considering the EVs’ cargo, small RNAs account for a large proportion of cargoes in EVs, and act as one of the critical post-transcriptional regulators in cell-to-cell communication. Among them, miRNAs are the most studied and their regulatory roles in host–pathogen interactions are increasingly clear. HPCs are a kind of stem cells with potentially bidirectional differentiation ability and interact with hepatic stellate cells during liver injury. The activation of HPCs play an important role during S. japonicum-induced liver fibrosis. We used microarrays to detail the gene expression of SEA-EVs and CON-EVs derived from HPC cell line, LE/6. LE/6 was selected and cultured with soluble egg antigen (SEA) from S. japonicum or PBS for 24hours. Then cell culture medium from LE/6 was harvested and stored at -80℃ until isolation of EVs. Isolated EVs were collected to detect the miRNA expression.