Functional remodelling of perinuclear mitochondria alters nucleoplasmic Ca2+ signalling in heart failure

Mitochondrial dysfunction in cardiomyocytes is a hallmark of heart failure (HF) development. Although initial studies recognized the importance of different mitochondrial subpopulations, there is a striking lack of direct comparison of intrafibrillar (IF) vs. perinuclear (PN) mitochondria during the development of HF. Here, we use multiple approaches to examine the morphology and functional properties of IF vs. PN mitochondria in pressure overload-induced cardiac remodeling in mice, and in non-failing and failing human cardiomyocytes. We demonstrate that PN mitochondria from failing cardiomyocytes are more susceptible to depolarization of mitochondrial membrane potential (ΔΨm), ROS generation and impairment in Ca2+ uptake compared to IF mitochondria at baseline and under physiological stress protocol. We also demonstrated, for the first time, that under normal conditions PN mitochondrial Ca2+ uptake shapes nucleoplasmic Ca2+ transients (CaTs) and limits nucleoplasmic Ca2+ loading. Loss ..., The following datasets are available:

A custom MATLAB script for semi-automated analysis of cytoplasmic, nucleoplasmic, or subcellular Ca²⁺ transients in electrically stimulated cardiomyocytes.

A step-by-step user guide to facilitate the use of the script.

Supplemental materials, including expanded Materials and Methods, Supplemental Table 1, and Supplemental Figure 1.

Data supporting Figures 1–6, including full-resolution images used in the figures and GraphPad files with individual data values and corresponding statistical analyses.

, , # Data from: Functional remodelling of perinuclear mitochondria alters nucleoplasmic Ca2+ signalling in heart failure

[https://doi.org/10.5061/dryad.pvmcvdnn9](https://doi.org/10.5061/dryad.pvmcvdnn9)

## Description of the data

The following files are available:

1. A custom MATLAB script for semi-automated analysis of cytoplasmic, nucleoplasmic, or subcellular Ca²⁺ transients in electrically stimulated cardiomyocytes (file format: MATLAB code; file name: *Script for semi-automated CaTs analyses*).
2. A step-by-step user guide to facilitate the use of the script (file format: docx; file name: *Detailed Instructions for Using the MATLAB Script*).
3. Data supporting Figures 1–6 (each in individual folder named: *Figure 1*, *Figure 2*..., *Figure 6*), including full-resolution images used in the figures (file format: jpg, folder name: *Full resolution images*) and GraphPad files with individual data values and corresponding statistical analyses (file format: GraphPad Prism Project; file...