Affinity matured anti-VEGF-C scFvs possess a higher affinity.
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https://figshare.com/articles/dataset/_Affinity_matured_anti_VEGF_C_scFvs_possess_a_higher_affinity_/508000
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(A, B) ELISA analysis of bacterial supernatant from randomly picked affinity matured clones after 1 to 3 rounds of selection on biotinylated (A) P. pastoris-derived or (B) mammalian cell-derived ΔNΔC-VEGF-C. (C) BIAcore profiles of monomeric affinity matured anti-VEGF-C scFvs. Monomeric fractions of protein-A purified scFv were prepared by FPLC and injected as 2-fold dilution series on a streptavidin-sensorchip coated with 2000 RU biotinylated ΔNΔC-VEGF-C derived from mammalian cells.
创建时间：
2010-08-02
