NOG21 genome sequence

We replaced the native glycolytic pathways in Escherichia coli with the previously designed non-oxidative glycolysis, which bypasses initial C3 formation and directly generates stoichiometric amounts of C2 metabolites, using rational design, genome editing, and evolution. NOG21 grows aerobically in glucose minimal medium, but can ferment anaerobically to products with nearly complete carbon conservation. NOG21 was sequenced using the Illumina MiSeq by the High Throughput Genomics Core, Academia Sinica, Taiwan.