SPRK1 shRNA and overexpression followed by RNA-seq in glioblastoma cells

SRPK1 is an SR (serine-arginine) protein kinase with an emerging role as a therapeutic target. SRPK1 phosphorylates the SR family of core spliceosomal components and accessory splicing factors, representing a pivotal regulator of splicing events in human cells. Because alternative splicing of mRNAs in the nervous system occurs at high frequency, the imbalance of mRNA isoforms can affect the function of important neural proteins. We used this RNA-seq dataset to classify splicing events upon genetic modulation of SRPK1, in glioblastoma cells. Overall design: We performed transient transfection of the SRPK1 protein kinase for overexpression and knockdown conditions, in T98G glioblastoma cells and untreated cells, described here as NT. We performed 2 biological replicates of each condition.