Temporal effects of sympathetic denervation on aortic remodeling and rupture in experimental abdominal aortic aneurysm

Abdominal aortic aneurysm (AAA) is a life-threatening condition with no effective pharmacologic therapies, and increasing evidence suggests that the sympathetic nervous system (SNS) contributes to disease progression. However, the temporal effects of SNS modulation on AAA stability remain unclear. To investigate the impact of SNS modulation on aneurysm outcomes, we induced AAAs in male ApoE-/- mice via continuous subcutaneous infusion of angiotensin II (AngII). Using 3D light sheet microscopy, we found that ruptured AAAs exhibited the highest sympathetic innervation in experimental aneurysms. Sympathetic denervation was performed using intraperitoneal 6-hydroxydopamine, administered either serially (before and during AAA induction) or at a delayed time point (after aneurysm formation). Mice were monitored for mortality, and surviving animals were evaluated at day 28 for aortic morphology, nerve density, vascularization, and inflammation. Both serial and delayed denervation significantly..., , # Temporal effects of sympathetic denervation on aortic remodeling and rupture in experimental abdominal aortic aneurysm

Dataset DOI: [10.5061/dryad.9zw3r22tp](10.5061/dryad.9zw3r22tp)

## Description of the data and file structure

AJP_Supplementary_DATA.docx:

* Table S1: Key experimental reagents and materials.

AJP_Supplementary_FIGURES.docx:

* Figure S1: Correlational analysis of nerve fiber density to lumen diameter of No Denervation, Delayed Denervation, and Serial Denervation groups.
* Figure S2: Representative immunohistochemical staining of aorta and vena cava after vehicle only (top left) and one week after 6-OHDA (top right). Representative immunohistochemical staining of mesenteric branch artery two weeks after 6-OHDA (bottom left) and four weeks after 6-OHDA (bottom right).

Supplemental Video 1 – 6: 3D light sheet microscopy image stack of tissue-cleared aortic walls from patients undergoing elective open AAA repair. SMA (red) highlights aortic smooth muscle, and TH (gr...,