Differential expression of RNAs in E9 tuft rostrums with NTDs

Purpose: Identify genes and pathways affected in tuft embryos with NTDs Results: Expression of genes associated with neural tube closure and components of non-canonical WNT signaling/PCP pathways were affected Conclusions: TET1 regulates genes associated with neural tube closure RNA pooled from the rostrums of E9 (18-22 somites) tuft/tuft embryos with NTD compared with respective wildtype background strain研究目的：鉴定携带神经管缺陷（Neural Tube Defects, NTD）的tuft/tuft纯合突变胚胎中受影响的基因与信号通路。 实验结果：与神经管闭合过程相关的基因，以及非经典WNT信号通路/平面细胞极性（PCP）通路的组成成分的表达水平均出现异常改变。 研究结论：TET1可调控与神经管闭合相关的基因。 实验样本：本研究的测序RNA样本取自胚胎发育第9天（E9，体节数18-22对）的携带NTD的tuft/tuft纯合突变胚胎的吻部组织，对照样本为对应遗传背景的野生型品系胚胎。