in vitro rumen fermentation Raw sequence reads

The objective of this study was to investigate the impact of the use of waste from essential oil extraction on the rumen microbiota, during the in vitro fermentation process. We used the wastet obtained from a supercritical CO2 extraction of Mint essential oil (Mint EO). Each fermentation was tested in 3 consequent incubations, and each incubation is made using 50 mL of rumen fluids, collected from 3 different dairy cows, mixed with 100 mL of culture medium and supplemented with 1 g of different matrix. As controls, one bottle from each cow was fermented with only medium and rumen fluid (rumen 24h), and another one with grass hay as supplement (CTRL). The raw Mint before essential oil extraction, the product obtained from the extraction, and the waste from the extraction were analyzed after addition as supplement to rumen fluid.Each bottle was fermented for 24 hours. Subsequently, a sample was collected and processed for bacterial DNA extraction and sequencing by Illumina NGS methodology.