Alteration of global transcription by the phytochemical Kaempferol in Acinetobacter baumannii AB5075

We perform RNA-seq comparing a treatment with 0.375 mM Kaempferol to a mock treatment (DMSO) in A. baumannii AB5075 in order to describe the alterations in global transcription produced by this phytochemical. A. baumannii AB5075 cultures were grown in LB medium in the presence of 0.375 mM Kaempferol or a DMSO control (3 biological replicates per condition) until reaching mid-exponential phase. At this point, cells were harvested, treated with RNAlater for preservation of total RNA and stored at -80 C. After that, total RNA was extracted from each sample. As a result, 99 genes appeared upregulated in the presence of Kaempferol whereas 18 were downregulated. In highlights, the gene functional groups showing the strongest transcriptional alterations were those related to iron acquisition, siderophore biosynthesis and iron transport genes. Overall design: Three independent replicates of A. baumannii AB5075 were grown to mid-exponential phase in cation-adjusted Muller-hinton (pH 7.2) medium supplemented with 0.375 mM Kaempferol and compared to three independent replicates grown in the same conditions but receiving a mock treatment (equivalent volume of DMSO).