Knockdown of ASD risk genes leads to a shared transcriptional signature

RNAseq analysis of shRNA-mediated knockdown of chromatin modifiers associated with Autism Spectrum Disorder Overall design: E16.5 primary cortical neurons were cultured for 5 days before infection by shRNAs targeting one of Ash1L, Chd8, Crebbp, Ehmt1, Nsd1, Gtf2i, or Luciferase. Neurons were cultured for 5 additional days before RNA was collected.