Redistribution of EC-SOD due to the R213G SNP reveals differential RNA-seq profile of recruited alveolar macrophages and accelerated apoptosis in response to bleomycin

A known human SNP in the matrix-binding domain of extracellular superoxide dismutase (EC-SOD), with an arginine to glycine substitution at position 213 (R213G), redistributes EC-SOD from the matrix into extracellular fluids. We reported that, following bleomycin (bleo), knock-in mice harboring the human R213G SNP (R213G mice), compared to wild-type (WT) littermates exhibit enhanced resolution of inflammation and protection against fibrosis. We tested the hypothesis that the EC-SOD R213G SNP promotes resolution via accelerated apoptosis of recruited alveolar macrophage (recAM). Overall design: RNA-seq of alveolar macrophages populations in response to bleomycin