aThe total volume of PCR reaction was 25 µl and the PCR conditions were as follows: 50°C for 2 min, 95°C for 10 min, followed by 40 cycles of 95°C, 15 s for denaturation and 60°C, 1 min for annealing

NIAID Data Ecosystem20

Characteristics of the primers used for SYBR green Q-PCR assays.

分子生物学

PCR技术

*Letters F and R after the primer name indicate forward and reverse orientation, respectively. †Theoretical melting temperature (Tm) calculated using the MWG Oligo Property Scan (MOPS).

NIAID Data Ecosystem60

Mutations in glpT of ten randomly chosen spontaneous mutants of P. aeruginosa PA14 selected from in vitro and in vivo experiments.

基因突变分析

功能基因组学

Mutants were arranged according to the nucleotide position using the A of the ATG of the ORF as reference.

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