SRC phosphorylates RUNX1

Based on studies in developing mouse megakaryocytes and T cells, SRC phosphorylates RUNX1 at seven tyrosine residues in the negative regulatory domain (Y254, Y258, Y260, Y376, Y379, Y380 and Y387). Endogenous human RUNX1 is tyrosine phosphorylated, and tyrosine residues in murine Runx1 that are phosphorylated by Src are conserved in human RUNX1. SRC-mediated phosphorylation interferes with binding of RUNX1 to GATA1, thus negatively regulating differentiation of hematopoietic progenitors. SRC-mediated phosphorylation promotes association of RUNX1 with the SWI/SNF complex (Huang et al. 2012).

基于对小鼠巨核细胞和T细胞发育的研究，Src在负调控域（Y254、Y258、Y260、Y376、Y379、Y380和Y387）的七个酪氨酸残基上磷酸化RUNX1。内源性人源RUNX1发生酪氨酸磷酸化，且被Src磷酸化的小鼠Runx1中的酪氨酸残基在人类RUNX1中得以保留。Src介导的磷酸化干扰RUNX1与GATA1的结合，从而负向调控造血祖细胞的分化。Src介导的磷酸化促进RUNX1与SWI/SNF复合物的结合（Huang等，2012年）。