CCDB_6348.jpg

Single computed slice through a double tilt electron tomogram of a
lamellar structure observed in HeLa cells transfected with mutant
connexin 50, imaged using intermediate voltage electron microscopy, and
labeled using the tetracysteine-ReHAsH system and fluorescence
photoooxidation. The concentric double membrane layers of the aggregate
appear to form "onion skin" sheets that do not form a closed structure
along the long axis. The differential staining between the outside and
inside is most likely due to impeded diffusion of DAB into the interior
of these accumulations.

对HeLa细胞中转染突变型connexin 50所观察到的层状结构的双倾斜电子断层扫描的单一计算切片进行成像，采用中电压电子显微镜进行成像，并使用四硫代半胱氨酸-ReHAsH系统及荧光光氧化法进行标记。该聚集体呈同心双层膜结构，似形成“洋葱皮”状薄片，沿长轴方向未形成封闭结构。外侧与内侧之间的差异染色，最可能是由于DAB物质难以扩散进入这些积累物内部所致。