Sequencing of human B cells in Lyme disease. Homo sapiens

Lyme disease (Borrelia burgdorferi infection) is increasingly recognized as a significant source of morbidity world-wide. Here, we investigated B cell responses to Lyme disease through molecular identifier-enabled antibody heavy chain sequencing of bulk B cells and single-cell paired-chain antibody sequencing of sorted blood plasmablasts. We identified differences in antibody repertoire characteristics among clinical subsets of Lyme disease patients as well as characteristics that distinguish Lyme disease patients from healthy controls.