Hierarchal classification of bacterial/archaeal (16S) and fungal (ITS) ASVs detected in soil samples based on the 16S rRNA SILVA database and the ITS UNITE database, respectively.
Four ITS reference sequence databases (D1–D4) were used to train the QIIME2 q2-feature-classifier. Database D1 includes 196,344 fungal ITS reference sequences from the Unite database37, while the D2 d
UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases
Supplementary Material 3: Supplementary Data 2. Taxonomic annotation of 2021 sargassum samples based on mitochondrial genome and 16S gene BLASTn comparisons with databases.
