Functional analysis of the NucS/EndoMS of the hyperthermophilic archaeon Sulfolobus islandicus REY15A

We report Over-expression of the wild type SisEndoMS in S. islandicus resulted in retarded growth and abnormal cell morphology. Transcriptomic analysis revealed that SisEndoMS overexpression led to upregulation of distinct sets of genes including the CRISPR-Cas IIIB system, methytransferases, and glycosyltransferases, which are mainly localized to a specific segment in the chromosome. RNA Seq/Transcriptomics analysis of SisEndoMS wild type overexpression strain and SisEndoMS catalytic and DNA binding mutant overexpression strain