Transcriptome Data Analysis of Artocarpus heterophyllus stem and leaves

Artocarpus heterophyllus is an important woody plant in tropical regions, and has high economic value. This test used normal and chlorophyll deficient mutant of A.heterophyllus seedlings as the research materials. The transcriptome of stem and leaves have been sequenced by PacBio Sequel, finally got 95701 isoforms. After cluster and polish, using NR, NT, GO, KOG, KEGG, Swissprot and Interpro 7 databases for functional annotation, a total of 93367 transcripts were annotated. There were 92 187 (NR), 90 326 (NT), 74 548 (Swissprot), 75 396 (KEGG), 77 022 (KOG), 81 906 (Interpro) and 65 500 (GO) transcripts annotated in the seven databases respectively. In addition, a total of 85091 CDS and 55608 SSR sequences were detected, 4753 transcription factors, 1667 transcriptional regulators and 4710 receptor-like kinases were predicted. The results of this study could provide a reference template for the splicing of second-generation sequencing data and lay a foundation for subsequent transcriptome studies.