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RNA sequencing of T helper cell type 2, with CRISPR KO. RNA sequencing of T helper cell type 2, with CRISPR KO

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NIAID Data Ecosystem2026-03-10 收录
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T cells were extracted from spleens of Cas9 mice and activated in vitro with CD3/28 on day 0. They were spinoculated with retroviruses containing sgRNA on day 1. On day 2 and forwarded they were treated with puromycin. On day 4, dead cells were removed by low-G spin and the remaining cells measured by a variant of SMART-seq2

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2017-12-13
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