Pluripotency-Independent Induction of Human Trophoblast Stem Cells from Fibroblasts [RRBS]

RRBS analysis revealed similarity/dissimilarity between human blastocyst derived trophoblast stem cells (hbdTSCs), human induced trophoblast stem cells (hiTSCs), human foreskin fibroblasts (HFFs) and pluripotent stem cells (hESCs, hiPSCs) HFFs reprogrammed into hiTSC lines by transient ectopic expression of GATA3, OCT4, KLF4, MYC (GOKM) were subjected to RRBS-protocol to assess differentially methylated regions, along with samples of HFFs, hbdTSCs, hESCs and hiPSCs