Differential transcriptomic expression of an imprved astaxanthin production

The differential expression of the high-yield astaxanthin Phaffia rhodozyma BPAX-A1 with the wild-type Phaffia rhodozyma CBS6938 was obtained and analysed to understand metabolic changes leading to an improved astaxanthin production. Overall design: We performed fermentations in instrumented fermenters with wild-type and mutant strains (three biological replicates with each strain) and samples were obtained at the late exponential growth phase for RNA-seq analysis- this phase presented the highest astaxanthin production rate suggesting to be the most informative to explain metabolic changes leading to an improved astaxanthin production in the mutant strain.