Effect of 3-deazaadenosine on the H3K36me3 chromatin landscape during senescence [ChIP-seq]

We treated IMR90 ER:RAS cells with 4OHT to induce RAS activation or with vehicle (DMSO) as a non-senescent control. At the same time, cells were treated with 10 µM 3-deaazadenosine. 3-deazaadenosine is a SAM-dependent methyltransferase inhibitor. H3K36me3 is a mark enriched at actively transcribed genes. Our previous experiments showed that treatment with 3-deazaadenosine prevents senescence and reduces overall levels of H3K36me3. By performing ChIP-seq, we have unveiled that treatment with 3-deazaadenosine prevents deposition of H3K36me3 in genes essential for the secretory phenotype of senescent cells. Examination of H3K36me3 under 3 different treatments: non-senescent cells (DMSO), senescent cells (4OHT), 3DA cells (4OHT + 3DA).