Genetic Dissection of Photosynthetic Organs Contribution to Seed Oil Accumulation in Arabidopsis seeds. Genetic Dissection of Photosynthetic Organs Contribution to Seed Oil Accumulation in Arabidopsis seeds

We report the application of RNA-seq technology for highthroughput profiling of photosynthetic and non-photosynthetic seeds of Arabidopsis chlorophyll synthase mutant seeds. By generating over 21 GB of sequence data from these seeds, we showed that genes involved in oil accumulation in non-photosynthetic seeds were significantly induced compared to photosynthtic seeds. Additionally we found that genes involved in the plastidal oxidative pentos phosphate pathway were significantly upregulated in the non-photosynthetic seed opposite to photosynthetic seeds. Overall our RNA-seq analysis revealled the genes and pathway interaction underpinining oil accumulation in non-photosynthetic seeds. Overall design: Examination of segregating green (photosynthetically-competent) and non-green (photosynthetically-non-competent) seeds in siliques of an Arabidopsis chlorophyll synthase mutant.