Persistent TCR clones infiltrating the transplanted hand.

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“>Dom” Indicates that the specified sequence was the dominant sequence revealed by bulk sequencing of the whole family
“ND” Not Done
*Obtained from first biopsy on Day 751
**Obtained from duplicate biopsy on Day 751
Any BV family demonstrating a peak expansion at more than one time point was subjected to bulk sequencing of the uncloned PCR product for the whole family. If no clearly dominant sequence was obtained, clonal sequencing (TOPO-TA) of at least 12 clones was performed for that family. For each listed peak and detected CDR3 sequence corresponding to that peak size, whether that sequence was the dominant sequence seen in bulk sequencing or the frequency of that sequence within clonal sequences is given for the indicated times after transplantation. The final column gives the minimum duration of each clonal sequence (time between first and last detection).