Efficient Non-integrating and Growth Factors-Free Culture System for Generation of Human Induced Pluripotent Stem Cells via Activation of Glycolysis

we found that a dual-specificity tyrosine phosphorylation-regulated kinase inhibitor ID-8 robustly enhanced human somatic cell reprogramming by upregulation of PDK4 and activation of glycolysis. Furthermore, we identified a novel growth factor-free hiPSC generation system using ID-8/KGN (IK). IK combined with Low-dose bFGF supported the long-term expansion of human pluripotent stem cells. Also, IK-iPSCs showed high pluripotency and normal karyotype. Our studies provide a novel growth factor-free culture system to facilitate generation of hiPSCs for multiple application in regenerative medicine. Examination of ID-8 promote reprogramming in fibroblast and control group E8 and E6 (E8 without bFGF,TGFb)