Gene expression profile at single cell level of phagocytic myeloid cells in autochthonous lung cancer model. Gene expression profile at single cell level of phagocytic myeloid cells in autochthonous lung cancer model

Phagocytosis is a critical process undertaken by myeloid cells in the tumor microenvironment. We used single-cell RNA-seq (scRNAseq) to investigate molecular and cellular consequences of neoplastic cell engulfment using a tdTomato genetically engineered mouse model. Overall design: CD45-positive and CD45-negative populations from the lung tissue of SPC-creER KRAS-G12D mice were isolated by Fluorescence-activated cell sorting (FACS) analyzed using scRNAseq with antibody-based cell hashing.