Novel perspectives on TRM signature and biology

The goal of this study was to better understand the development of resident memory T cells (Trm) and central memory T cells (Tcm). To do this, we performed single-cell RNAseq on antigen-activated OT-1 T cells at serial timepoints collection from both skin and lymph node across 2 months post-vaccination. CD90.1 OT-1 T cells were adoptively transferred to a CD90.2 mouse, which was then infected with VACV-OVA. Skin and lymph node samples were then collected and digested at different timepoints. OT-1 T cells were sorted from these timepoints and used for single-cell RNAseq