Gene expression profile at single cell level of myeloid cells from juxta tumor (JT) and tumor (T) samples from treatment naive triple negative breast cancer (TNBC) patients

Myeloid cell subpopulation comprises denditric cells (DC), monocytes and macrophages. We used single cell RNA sequencing (scRNA-seq) to analyze the heterogeneity of the myeloid cells, particularly to study tumor-assocaited macrophages in TNBC patients. Overall design: JT and T myeloid cells from TNBC patients were isolated by FACS-sorting, collecting HLADR+CD11C+ double positive cells, excluding from the sorting strategy CD3, CD19, CD56 and dead cells. The collected cells were analyzed using scRNAseq.