Details of roadkill samples that tested positive for rabies using the LN34 real-time RT-PCR assay. Presence of lyssavirus RNA is indicated by average LN34 Ct value (LN34) ≤ 35, where lower numbers generally indicate higher RNA levels. Actin Ct values (Actin) indicate presence of host β-actin mRNA and are indicative of sample quality, where high Ct values may indicate poor sample quality or RNA degradation. A full description of how questionnaires were filled out is described in the methods. Temperature (Temp).

Details of roadkill samples that tested positive for rabies using the LN34 real-time RT-PCR assay. Presence of lyssavirus RNA is indicated by average LN34 Ct value (LN34) ≤ 35, where lower numbers generally indicate higher RNA levels. Actin Ct values (Actin) indicate presence of host β-actin mRNA and are indicative of sample quality, where high Ct values may indicate poor sample quality or RNA degradation. A full description of how questionnaires were filled out is described in the methods. Temperature (Temp).