Genome-wide map of histone H3 lysine 36 tri-methylation in wild type mouse myocytes and myocytes bearing a muscle-specific H19/Igf2 enhancer deletion. Mus musculus

We report a genome-wide study of the role of a muscle-specific Igf2/H19 transcriptional enhancer in global histone modifications in primary mouse myocytes. We generated 1.7 billion bases of sequence from DNA purified by the chromatin immunoprecipitation of H3K36me3 in wild type myocytes and myocytes bearing a deletion of the enhancer. Because H3K36me3 is reported to mark transcribed gene regions, we generated these data to develop a list of genes that are directly or indirectly regulated by this muscle enhancer. This study provides additional insight into how this muscle-specific enhancer potentially regulates a transcriptional network in mouse skeletal myocytes. Overall design: Examination of 1 histone modification in a wild type myocyte line and a mutant myocyte line